FIG. 3.

Phylogenetic tree indicating the relationship between rlMctP and related permeases. A phylogenetic tree was constructed from the amino acid sequences of rlMctP and a number of related transporters using Vector NTI Suite (version 6), which uses the ClustalW algorithm, and Treeview (version 1.6.1). Protein designations and accession numbers are as follows: scCAB94614, S. coelicolor A3 putative permease (CAB94614); Ta0300, T. acidophilum conserved hypothetical protein (CAC11445); ST0635, S. tokodaii conserved hypothetical protein (BAB65633); ssC02007, S. solfataricus conserved hypothetical protein c02007 (CAA69485); bsYodF, B. subtilis YodF (C69903); TVN0146, T. volcanium proline permease (BAB59289); TVN1300, T. volcanium Na⁺/pantothenate symporter; bsYhjB, B. subtilis metabolite permease (B69833); bcYhjB-like, B. cereus YhjB-like protein (AF387344); ecPanF, E. coli Na⁺/pantothenate symporter (P16256); hiPanF, Haemophilus influenzae Rd Na⁺/pantothenate symporter (P44963); ecPutP, E. coli Na⁺/proline symporter (P07117); hiPutP, H. influenzae Rd Na⁺/proline symporter (P45174); bsOpuE, B. subtilis osmoregulated Na⁺/proline symporter (O06493); ppPpa, P. aeruginosa phenylacetic acid transporter (CAA94864); hsMCT1 to -6, human monocarboxylic acid transporters 1 to 6 (P53985, AF049608, O15427, O15374, O15375, and O15403); ssc01003, S. solfataricus orf c01003 (CAA69453); rlDctA, R. leguminosarum C₄-dicarboxylate transporter (Q01857); rmDctA, S. meliloti C₄-dicarboxylate transporter (P20672); ecDctA, E. coli C₄-dicarboxylate transporter (P37312); ps3Acp, thermophilic bacterium PS3 alanine carrier protein (D12512); ahDagA, Alteromonas haloplanktis d-alanine/glycine permease (M59081); ssLctP, S. solfataricus l-lactate permease (CAA69452); bsLctP, B. subtilis l-lactate permease (BAA21776); ecLctP, E. coli l-lactate permease (AAC76627); hiLctP, H. influenzae Rd l-lactate permease (AAC22871).