FIG. 5.
Uptake of lactate and pyruvate by strains of R. leguminosarum. Uptake of 5 μM (0.125 μCi) l-[U-14C]lactate (A) and [1-14C]pyruvate (C) was assayed by the rapid filtration method for different R. leguminosarum strains grown in minimal medium (AMS) with added glucose (10 mM) plus ammonium chloride (10 mM). The apparent specificity of lactate (B) and pyruvate (D) uptake by strain 3841 was determined by the addition of competing solutes to a final concentration of 0.5 mM. Data shown are the means of at least three independent experiments. HB, hydroxybutyrate.