TABLE 2.
Growth of mutant strains containing various plasmids on minimal medium (AMA) containing either alanine or pyruvate and ammonia as a sole C and N source
| Plasmid | Growth of strainb
|
|||
|---|---|---|---|---|
| RU1180 (mctP::Tn5) | RU1581 (mctP::GJ) | RU1580 (mctS::GJ) | RU1582 (mctR::GJ) | |
| pRK415 | ± | ± | + | + |
| pRU785 (mctRP) | +++ | ND | ND | ND |
| pRU788 (mctRP)a | +++ | ND | ND | ND |
| pRU739 (mctP)a | +++ | ND | ND | ND |
| pRU747 (mctP) | + | ND | ND | ND |
| pRU947 (mctP) | +++ | +++ | ND | ND |
| pRU948 (mctP)a | ++ | +++ | ND | ND |
| pRU950 (mctS)a | ND | ND | ± | ND |
| pRU951 (mctR)a | ND | ND | ND | +++ |
| pRU953 (mctR) | ND | ND | ND | + |
| pRU952 (mctSR)a | ND | ND | +++ | +++ |
| pRU974 (mctSR) | ND | ND | +++ | +++ |
a
The mct gene is under the control of the lac promoter, which is constitutive in R. leguminosarum. Thus, no IPTG was required to induce the expression of the cloned genes.
b
There was no discernible difference between growth of strains on minimal medium with alanine (20 mM) or pyruvate (20 mM) and ammonium chloride (10 mM). Symbols: ±, negligible growth; +, positive for growth; +++, strongly positive for growth. ND, not determined.