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. 2002 Oct;184(19):5240–5250. doi: 10.1128/JB.184.19.5240-5250.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 4. — Vfr overexpression downregulates FleQ, thereby reducing motility. (A) Motility assay conducted on 0.3% agar plates containing carbenicillin and various IPTG concentrations, as indicated. Spots a and b, PAK harboring the vector control pMMB66EH and the vfr overexpression plasmid pWNP28, respectively. (B and C) Western analysis of the strains used in the motility assay with different IPTG concentrations and with anti-FleQ (B) and anti-Vfr (C) antibodies. Lanes1 to 4 contained lysates from cultures of PAK harboring pMMB66EH grown with 0, 0.01, 0.1, and 1.0 mM IPTG, respectively. Lanes 5 to 8 contained lysates from cultures of PAK harboring pWNP28 grown with 0, 0.01, 0.1, and 1.0 mM IPTG, respectively.