TABLE 3.
Effect of vfr overexpression on the activity of the wild-type fleQ promoter and its mutagenized derivative
| Promoter-lacZ fusion (chromosomal insertion) | β-Galactosidase activities (Miller units) in the following strains:
|
|||
|---|---|---|---|---|
| PAK(pMMB66EH) (vector) | PAK(pWNP28) (vfr) | PAO(pMMB66EH) (vector) | PAO(pWNP28) (vfr) | |
| fleQp-lacZ | 1,007 ± 15a | 253 ± 7 | 2,649 ± 37 | 1,267 ± 6 |
| fleQpmutV-lacZ | 1,339 ± 4 | 680 ± 49 | 3,341 ± 76 | 3,005 ± 59 |
| Promoterless lacZ | 33 ± 3 | 54 ± 1 | 92 ± 60 | 150 ± 32 |
a
The values are means ± standard deviations.