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(A) Gel shift assay to show binding of MBP-FleQ to 588-bp flhA promoter. Lane 1, free DNA probe; lanes 2 to 5, DNA incubated with 0.5, 2, 5, and 15 μM MBP-FleQ. (B to D) Gel shift assays with 332-bp fliE promoter (B), 412-bp fliL promoter (C), or 355-bp fleSR promoter (D). Lane 1, free DNA probe; lanes 2 to 6, 0.5, 1, 2.5, 3.75, and 5 μM MBP-FleQ; lane 7 (panels B and D only), 7 μM MBP-FleQ.
