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Primer extension analysis of P. aeruginosa RNAs. (A) flhA promoter using primer JJ26; (B) fliE promoter using primer JJ27; (C) fliL promoter using primer JJ48. The TSSs are marked as +1 and are shown with an asterisk on the sequence readout from the sequencing lanes using the same primers. RNA was prepared from PAK wt or a fleQ mutant strain.
