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(A) Gel shift analysis using the mutagenized flhA promoter (mutated at its FleQ binding site). Lanes 1 to 4 have wt flhA promoter and lanes 5 to 8 have the mutated flhA promoter. Lanes 1 and 5, free DNA probe; lanes 2 and 6, 2 μM MBP-FleQ; lanes 3 and 7, 5 μM MBP-FleQ; lanes 4 and 8, 7.5 μM MBP-FleQ. (B) β-Galactosidase assay results comparing the promoter activities of the mutagenized flhA promoter with the wt promoter.
