TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype | Source |
|---|---|---|
| E. coli strains | ||
| DH5α | hsdR recA lacZYA f80 lacZDM15 | GIBCO-BRL |
| XL1 Blue | recA1 endA1 gyrA96thi-1 hsdR17 supE44 relA1 lac[F′ proAB lacIqZDM15 Tn10 (Tetr)] | Stratagene |
| P. aeruginosa strains | ||
| PAK | Wild-type clinical isolate | D. Bradley |
| PAK-Q | PAK fleQ::Gmr | 1 |
| PAK-NIG | PAK rpoN::Gmr | 15 |
| Plasmids | ||
| pDN19lacΩ | Promoterless lacZ oriV oriT Tetr Strr Ω fragment | 30 |
| placΩA | pDN19lacΩ containing a 588-bp Eco-Bam fragment of the flhA promoter region | 9 |
| placΩA(Δ1-362bp) | pDN19lacΩ containing a 231-bp Eco-Bam fragment of the flhA promoter region | This study |
| placΩE | pDN19lacΩ containing a 332-bp Eco-Bam fragment of the fliE promoter region | 3 |
| placΩS | pDN19lacΩ containing a 355-bp Eco-Bam fragment of the fleSR promoter region | 1 |
| placΩL2 | pDN19lacΩ containing a 412-bp Eco-Bam fragment of the fliLMNOPQ promoter region | This study |
| pUC-SR | pUC19 containing insert of placΩS | This study |
| pUC-E | pUC19 containing insert of placΩE | This study |
| pBSK-A | pBSK containing insert of placΩA | This study |
| pBSK-L2 | pBSK containing insert of placΩL2 | This study |
| pBSK-AmutA | pBSK-A but with mutA in the FleQ binding site | This study |
| placΩ-AmutA | placΩA but with mutA in the FleQ binding site | This study |
| pIH1119 | oriV in pGEM3Zf(+) | NEBa |
| pIH-fleQ | FleQ clone into Eco-Bam sites of pIH1119 to express MBP-FleQ | 10 |
a
NEB, New England Biolabs.