TABLE 2.
Primers used in this study
| Primer name | Primer sequencea |
|---|---|
| JJ2 | 5′ gatccATGGCGGGATCGGCAG 3′, part of BamHI site |
| JJ5 | 5′ gatccCGCATTTCCAGCATC 3′, part of BamHI site |
| JJ8 | 5′ gatccCGTTGAGGGCTGGTTGC 3′, part of BamHI site |
| JJ26 | 5′ GGTTGCTGCGCACATTGCTGATCAGTTGC 3′ |
| JJ27 | 5′ CCAGCATCAGACGATTGAACTCGACACCC 3′ |
| JJ48 | 5′ CTCTTGCCGCCGCTCGGCGCCCCCGCG 3′ |
| JJ37 | 5′ cccaaaggatccCAGGATCAGCTTCAGCTTG 3′, BamHI site incorporated |
| JJ38 | 5′ gatccCAGGATCAGCTTCAGCTTGCTCTTG 3′, part of BamHI site |
| JJ39 | 5′ CATTCCGGCGTtctcAGTTTGTTCGGC 3′ |
| JJ40 | 5′ GCCGAACAAACTgagaACGCCGGAATG 3′ |
| 5PfliLβgal | 5′ cccaaagaattcCTCGGGCGATGAGGAAC 3′, EcoRI site incorporated |
| JJ61 | 5′ cccaaagaattcAACCCTTTTCAATCAATGAATTG 3′ |
| RER62 | 5′ cccaaaggatccATGGCGGGATCGGCAGGG 3′ |
a
Lowercase letters denote nucleotides added or modified to facilitate restriction digestion or mutagenesis.