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Purification of AtzC
| Fraction | Total protein (mg) | Total activity (U)a | Sp act (U mg−1) | Yield (%) | n-Fold purification |
|---|---|---|---|---|---|
| Crude | 412 | 2,380 | 5.80 | 100 | |
| Ammonium sulfate | 86.9 | 1,020 | 11.7 | 42.6 | 2.0 |
| Phenyl column | 27.9 | 452 | 16.2 | 19.0 | 2.8 |
1 U of enzyme activity is defined as the conversion of 1 μmol of N-isopropylammelide to cyanuric acid and isopropylamine in 1 min.
