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Expression from the ilvIH promoter in vivo in strains having addition and deletion mutations. The specific activity of β-galactosidase (Miller units) (13) was measured in the indicated strains in cells grown in minimal medium lacking (−Leu) or containing (+Leu) leucine (50 μg/ml). Sites at which Lrp binds upstream of the promoter are represented in the cartoon as solid circles (dimer assumed to bind to one site). Arrow, start point for transcription; wt, wild type; Δ, deletion.
