TABLE 1.
Bacterial strains, phages, and plasmids used in this study
| Strain, plasmid, or phage | Relevant genotype | Source or reference |
|---|---|---|
| E. coli K-12 strains | ||
| CV981a | ara (lac-pro) thi | 13 |
| CV1042 | CV981-λQW156 | 20 |
| CV1327 | CV981-λQW223 | 20 |
| CV1555 | CV981-λSJ315 | This study |
| CV1556 | CV981-λSJ316 | This study |
| CV1557 | CV981-λSJ317 | This study |
| CV1558 | CV981-λSJ318 | This study |
| CV1559 | CV981-λSJ319 | This study |
| CV1560 | CV981-λSJ320 | This study |
| CV1561 | CV981-λSJ321 | This study |
| CV1562 | CV981-λSJ322 | This study |
| Plasmids | ||
| pRS415 | Contains promoterless lacZYA | 19 |
| pCV156 | pRS415 containing −332 to +29 of ilvIH region fused to lacZYA | 20 |
| pCV223 | pCV156 but Δ1, 2, 3, 4, 5, 6 | 20 |
| pCV315 | pCV156 but Δ1&2 | This study |
| pCV316 | pCV156 but 100-bp insertion | This study |
| pCV317 | pCV156 but Δ1&2, 100-bp insertion | This study |
| pCV318 | pCV156 but 2-bp insertion | This study |
| pCV319 | pCV156 but 4-bp insertion | This study |
| pCV320 | pCV156 but 6-bp insertion | This study |
| pCV321 | pCV156 but 8-bp insertion | This study |
| pCV322 | pCV156 but 10-bp insertion | This study |
| Phages | ||
| λRS45 | Contains promoterless lacZYA | 19 |
| λQW156 | λRS415 containing −332 to +29 of ilvIH region fused to lacZYA | 20 |
| λQW223 | λQW156 but Δ1, 2, 3, 4, 5, 6 | 20 |
| λSJ315 | λQW156 but Δ1&2 | This study |
| λSJ316 | λQW156 but 100-bp insertion | This study |
| λSJ317 | λQW156 but Δ1&2, 100-bp insertion | This study |
| λSJ318 | λQW156 but 2-bp insertion | This study |
| λSJ319 | λQW156 but 4-bp insertion | This study |
| λSJ320 | λQW156 but 6-bp insertion | This study |
| λSJ321 | λQW156 but 8-bp insertion | This study |
| λSJ322 | λQW156 but 10-bp insertion | This study |
a
Strain CV981 is strain P90C (CSH26) of Miller (13).