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. 2002 Oct;184(19):5293–5300. doi: 10.1128/JB.184.19.5293-5300.2002

TABLE 2.

Primers used in creating mutationsa

Mutation created Primer Position of 3′ end of primer Sequence of primer (5′ → 3′)
1 −320 GCAGAATTCCCCCATCAGTGGATG
4 b CCAGGGTTTTCCCAGTCACGACG
+2 2 −172 CCACAACTTAGCTAGCAATTTCTC
3 −151 GAGAAATTGCTAGCTAAGTTGTGG
+4 5 −172 CCACAACTTGCTAGCAGCAATTTCTC
6 −151 GAGAAATTGCTGCTAGCAAGTTGTGG
+6 7 −172 CCACAACTTAGCTAGCCAGCAATTTCTC
8 −151 GAGAAATTGCTGGCTAGCTAAGTTGTGG
+8 9 −172 CCACAACTTAAGCTAGCTCAGCAATTTCTC
10 −151 GAGAAATTGCTGAGCTAGCTTAAGTTGTGG
+10 11 −172 CCACAACTTAAGCTAGATCTCAGCAATTTCTC
12 −151 GAGAAATTGCTGAGATCTAGCTTAAGTTGTGG
+100 13 c TAGAGAAATTGCTGGTTGTCCCAATTCTTGTTG
14 c GGCTGAATCCCACAACTTAGGGTAAGTTTTCCGTATG
15 −142 CATACGGAAAACTTACCCTAAGTTGTGGGATTCAGCC
16 −174 CAACAAGAATTGGGACAACCAGCAATTTCTCTA
Δ1&2 17 −142 CGAGAATTCTAAGTTGTGGGATTCAGCC
Δ1&2, +100 18 c CGAGAATTCGTTGTCCCAATTCTTGTT
a

Inserted bases are shown in bold, and ensuing restriction sites are underlined. The numbering refers to the startpoint of transcription of the ilvIH operon (20).

b

This primer corresponds to sequences in lacZ in plasmid pCV156. Plasmid pCV156 contains the region from −332 to +29 of the ilvIH operon, followed by trp and lac sequences.

c

These primers correspond to sequences in the gfp gene, encoding green fluorescent protein.