TABLE 2.

Primers used in creating mutations^a

Mutation created	Primer	Position of 3′ end of primer	Sequence of primer (5′ → 3′)
	1	−320	GCAGAATTCCCCCATCAGTGGATG
	4	—b	CCAGGGTTTTCCCAGTCACGACG
+2	2	−172	CCACAACTTAGCTAGCAATTTCTC
	3	−151	GAGAAATTGCTAGCTAAGTTGTGG
+4	5	−172	CCACAACTTGCTAGCAGCAATTTCTC
	6	−151	GAGAAATTGCTGCTAGCAAGTTGTGG
+6	7	−172	CCACAACTTAGCTAGCCAGCAATTTCTC
	8	−151	GAGAAATTGCTGGCTAGCTAAGTTGTGG
+8	9	−172	CCACAACTTAAGCTAGCTCAGCAATTTCTC
	10	−151	GAGAAATTGCTGAGCTAGCTTAAGTTGTGG
+10	11	−172	CCACAACTTAAGCTAGATCTCAGCAATTTCTC
	12	−151	GAGAAATTGCTGAGATCTAGCTTAAGTTGTGG
+100	13	—c	TAGAGAAATTGCTGGTTGTCCCAATTCTTGTTG
	14	—c	GGCTGAATCCCACAACTTAGGGTAAGTTTTCCGTATG
	15	−142	CATACGGAAAACTTACCCTAAGTTGTGGGATTCAGCC
	16	−174	CAACAAGAATTGGGACAACCAGCAATTTCTCTA
Δ1&2	17	−142	CGAGAATTCTAAGTTGTGGGATTCAGCC
Δ1&2, +100	18	—c	CGAGAATTCGTTGTCCCAATTCTTGTT

^aInserted bases are shown in bold, and ensuing restriction sites are underlined. The numbering refers to the startpoint of transcription of the ilvIH operon (20).

^bThis primer corresponds to sequences in lacZ in plasmid pCV156. Plasmid pCV156 contains the region from −332 to +29 of the ilvIH operon, followed by trp and lac sequences.

^cThese primers correspond to sequences in the gfp gene, encoding green fluorescent protein.