TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| E. coli | ||
| DH5αphe | DH5α phe::Tn10dCm | 5 |
| S17-1 | C600::RP4-2(Tc::Mu)(Km::Tn7)thi pro hsdR recA Tra+ | 21 |
| R. sphaeroides | ||
| 2.4.1 | Wild type | W. Sistrom |
| CCOP1 | 2.4.1 derivative, ccoP::ΩTpr | 16 |
| JZ722 | 2.4.1 derivative, end of ccoN::Tn5TpMCS | 23 |
| JZ1678 | 2.4.1 derivative, ΔfnrL::ΩKmr | 22 |
| Plasmids | ||
| pRK415 | Tcr; Mob+lacZα IncP | 8 |
| pGEM-T | Apr; TA cloning vector | Promega |
| pCF1010 | Tcr; Smr; Spr; IncQ/IncP4, promoter cloning vector | 12 |
| pJR194 | pGEM-T::979 bp upstream of rdxB | This study |
| − 380 | pGEM-T::380 bp upstream of rdxB | This study |
| − 112 | pGEM-T::112 bp upstream of rdxB | This study |
| pUI1970 | pRK415::fnrL | 24 |
| pUI2803 | pRK415::4.7-kb BamHI + EcoRI containing ccoNOQP | 16 |
| pUI2805 | pRK415::4.2-kb PstI + BamHI containing rdxBHI′ | 16 |
| pJR103 | pRK415::4.9-kb PstI fragment containing rdxBHIS | This study |
| pJR235 | pRK415::8.3-kb BamHI + PstI containing ccoNOQP-rdxBHIS | This study |
| pJR471 | pRK415::2.9-kb EcoO109I + PstI containing rdxH'IS | This study |
| pJR473 | pRK415::8.1-kb BamHI fragment containing ccoNOQP-rdxBHI′ | This study |
| pJR316 | pCF1010::380 bp upstream of rdxB | This study |
| pJR319 | pCF1010::112 bp upstream of rdxB | This study |
| pJR320 | pCF1010::753 bp upstream of rdxH | This study |
| pJR321 | pCF1010::442 bp upstream of rdxI | This study |
| pJR322 | pCF1010::805 bp upstream of rdxS | This study |