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Levels of SpoIVFA, pro-σK, and σK during sporulation. (A) B. subtilis strain OR758 was induced to sporulate, samples were collected at the indicated times (hours) after sporulation was induced, and whole-cell extracts were subjected to Western blot analysis with antibodies against SpoIVFA. (B) The blot was stripped and reprobed with antibodies against pro-σK. (C) Quantification of the SpoIVFA (•) and σK (○) signal intensities from panels A and B, respectively.
