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Levels of SpoIVFA and pro-σK in mutants. B. subtilis strains were induced to sporulate, samples were collected at the indicated times (hours) after sporulation was induced, and whole-cell extracts were subjected to Western blot analysis with antibodies against SpoIVFA. (A) LK1 (spoIVBΔ::spc). (B) OR745 (spoIVFΔB::spc). (C) PY79 (spo+). (D) The blot in panel A was stripped and reprobed with antibodies against pro-σK.
