FIG. 6.
Effect of a protein synthesis inhibitor on pro-σK processing and on the levels of SpoIVFA and SpoIVFB-GFP. (A) B. subtilis strain OR758 was induced to sporulate, and the culture was split at T3 into portions to which nothing was added or Cm (200 μg/ml) was added immediately (Cm at 3 h) or after 30 min (Cm at 3.5 h). Shaking was continuous except when the culture was split and Cm was added. Samples were collected at the indicated times (hours) after sporulation was induced, and whole-cell extracts were subjected to Western blot analysis with antibodies against pro-σK. (B) Eightfold-longer exposure of part of the blot shown in panel A. (C) The blot was stripped and reprobed with antibodies against SpoIVFA. (D) The same samples used to produce the blot shown in panels A to C were used to produce another blot that was probed with antibodies against GFP to detect the SpoIVFB-GFP fusion protein.