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Depletion of topoisomerase I suppresses the anucleate phenotype of smc null mutants
| Relevant genotype (strain) | IPTG | % Anucleate cells (total no. of cells scored)a |
|---|---|---|
| Δsmc (RB35) | − | 13 (455) |
| Pspac-topA Δsmc (RB123) | + | 13 (807) |
| Pspac-topA Δsmc (RB123) | − | 0.1 (748) |
Cells were grown in defined minimal glucose medium at 30°C and scored for anucleate cells. Three independent experiments were performed. The cultures were grown from single colonies with 1 mM IPTG (+) or no IPTG (−).
