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. 2002 Nov;184(21):5987–5998. doi: 10.1128/JB.184.21.5987-5998.2002

FIG. 1.

FIG. 1.

(A) Restriction map and arrangement of the ORFs of vpma genes within the vpma locus of 55-5. Black arrows represent vpma genes and white arrows indicate ORFs unrelated to vpma genes. Lines above vpma ORFs indicate the locations of the vpma-specific PCR probes. A, AlwI; As, AseI; E, EcoRI; H, HindIII; P, PstI; X, XbaI. The location of a tRNA-lys gene is indicated by a black arrowhead. (B) Southern blot analysis of restricted DNA (1 μg) from PG2 clones hybridized with A4F. The tree schematic above each lane outlines the derivation of PG2 clones; each number represents a different clone which was either MAb 3B3-positive (+) or 3B3-negative (−). Lanes 1, 55-7; lanes 2, 55-5; lanes 3, 55-5-10; lanes 4, 55-5-10-4; lanes 5, 55-5-10-7. The restriction enzyme used is indicated below each panel. SD, λHindIII DNA standard.