TABLE 1.
Oligonucleotide sequences used in this study
| Name | Sequence (5′ to 3′)a |
|---|---|
| conF2 | cgcggatccAAGCTTAGTAAAATCAGTATAGAT |
| conF3 | CTGAACCATTAGGAGTAGTAAC |
| MAX2R | TTCTACCATATTGACTCCTATGT |
| Z3R | GACCTCCGCCCTGTGTGTCT |
| Z1F | cgcggatccCAAACAGATTCAACTCCGTCAAC |
| E1F | CCAAACTATTCAAGCCCAATTGAA |
| E1R | GTCAGATTCTGATTGTGCCGA |
| Z1R | aaactgcagTTATTCGTATTTAGGTAATAGTCTTC |
| E2F | GTCTGAAAATGATAAGAAATAAC |
| E2R | GCTCTAGTAAAAGGATTTGAACG |
| U1R | aaactgcagTCAACCTTAGATAAACCACCTAAC |
| U1F | cgcggatccGATAAAGAAGATAAGACAGGTGGTAG |
| UV2R | CTTGTGCCATTCTTTCAGGGG |
| UV1R | GGTTTTCCTGGTTGTTCTGTG |
| Y1R | gctctagaGATTAAACTTTTTTTACAGTAAATG |
| Y1F | ccggaattcAATGCAAACGCTGCAGAAAATG |
| YX1R | CTTYAAATTYTGGAGCTAAGCCTC |
| Y4R | TGACTGCCTTCTGCTGGAGT |
| X2R | TATTTTGACCTCTACCTTGTGTA |
| X1F | cgcggatccAAAGTAATGAAGGTCAATTACC |
| H4F | GAAACCCCAGAAGAAAGGGATG |
| X1R | aaactgcagGCTTAAGGATTTTTTAAAATGATG |
| W1R | aaactgcagAATAACTTTATCTAGTTCTATACC |
| H4R | CCTTAAAAATAGTGGTATAGAACTAGA |
| W3R | GTTGTTGCACTGTTACCATTACT |
| W1F | cgcggatccAATGGCGGAAATAGTAATGGTAAC |
| V2R | ATCTCAACGTATTATCGTTTTAC |
| V1R | gctctagattaTCCAGATGATGTTTCAACTTC |
| V1F | ccggaattcGTTGAGGAAGCAATTAAAACAGC |
| conR3 | GCAAATTAGTTGGTTTTTCAGC |
| conR2 | cgcggatccAAGCTTAATGATTTGTGCTATAAAA |
| A3F | AARTGYGGWGGWACWAMWRA |
| A4F-DIG | AARTGTGGTGGYACTAAAGA |
| 5′ leader1 | GGATAAATTTATGAAAAAATC |
| leader2 | ATGAAAAAATCAAADTTTKT |
a
Uppercase letters indicate target sequences, and lowercase letters indicate bases introduced to form restriction sites to aid in the cloning of subsequent PCR products.