TABLE 5.
Transfer frequencies of various pVT745 derivatives
| Donor | Plasmid | Recipient | No. of transconjugants/donora after:
|
|||
|---|---|---|---|---|---|---|
| 0.5 h on:
|
6 h on:
|
|||||
| RIF, KAN | RIF, SPT | RIF, KAN | RIF, SPT | |||
| VT745 | pDMG21A | ATCC 29522Rif | <10−7 | NA | 10−6 | NA |
| VT745 | pDGM21A | ATCC 29522RifrecA | ND | NA | 10−6 | NA |
| ATCC 29522 | pDGM21B | ATCC 700685Rif | ND | NA | 10−6 | NA |
| VT745 | pDMG27 | ATCC 29522Rif | 10−4 | NA | 10−3 | NA |
| VT745 | pDMG27 | ATCC 29522RifrecA | 10−4 | NA | 10−3 | NA |
| VT745 | pDMG28 | ATCC 29522Rif | <10−7 | <10−7 | 10−6 | NA |
| VT745 | pDMG30 | ATCC 29522Rif | 10−4 | 10−4 | 10−3 | 10−3 |
| VT745 | pDMG32 | ATCC 29522Rif | 10−6 | 10−6 | 10−5 | 10−5 |
a
The number of transconjugants per donor (average of at least two independent experiments) is shown. Selection of transconjugants was with RIF for the recipient strain and either KAN or SPT for the incoming plasmid. NA, not applicable; ND, not determined.