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Purified ATP-GLK from A. pernix (A) and recombinant ATP-GLK from transformed E. coli (B) as analyzed by SDS-PAGE (30). Protein was denatured in SDS and separated in 12% polyacrylamide slab gels (8 by 7 cm), which were stained with Coomassie brilliant blue R-250. (A) Lane 1, molecular mass standards; lane 2, native enzyme purified from A. pernix. (B) Lane 1, recombinant enzyme purified from E. coli (see Materials and Methods); lane 2, molecular mass standards.
