TABLE 1.

Purification of ATP-GLK from A. pernix

Purification step	Amt of protein (mg)	Activity (U)a	Sp act (U/mg)	Yield (%)	Fold purification
Cell extract	750	112	0.15	100	1
DEAE-Sepharose	100	60	0.6	53.6	4
ATP-agarose	0.37	13	35	11.6	233

^aEnzyme activity was measured at 90°C in the direction of G6P formation in a discontinuous assay with GPD as described in Material and Methods (1 U = 1 μmol of NADP⁺ reduced/min).