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Ion-exchange chromatography of the insecticidal compound of strain 407-1(Cry−)(Pig+). (a) Purified compound; (b) coinjection of the purified compound with 1.5 mg of β-exotoxin I standard; (c) 1.5 mg of β-exotoxin I standard. The ion-exchange chromatography was performed on a MonoQ column (Amersham Biosciences) in Bis-Tris, pH 6.0, with a gradient of sodium chloride.
