TABLE 2.
Complementation of pdaA with a plasmid containing the entire pdaA gene or the putative signal peptide-deficient pdaA genea
| Strain | Plate assay | Microscopic observation
|
Germination ratio (%) | ||
|---|---|---|---|---|---|
| No. of outgrowing spores | Spores | Vegetative cells | Sporulation ratio (%) | ||
| YFJSd (pHYCMfjS) | 5.57 × 107 | 2.85 × 108 | 10.2 × 107 | 73.6 | 19.5 |
| YFJSd (pHYCMΔSfjS) | 9.8 × 104 | 5.15 × 108 | 6.72 × 107 | 88.5 | 0.019 |
| YFJSd (pHYCM1) | 2.0 × 103 | 2.53 × 108 | 11.5 × 107 | 68.8 | 0.0008 |
| 168 (pHYCM1) | 7.15 × 107 | 2.94 × 108 | 6.40 × 107 | 82.1 | 24.3 |
a
YFJSd is a pdaA mutant. pHYCMfjS and pHYCMΔSfjS contained the entire pdaA gene and the putative signal peptide-deficient pdaA gene, respectively, downstream of the citM promoter region. pHYCM1 is a control plasmid (lacking the pdaA gene). Expression of the genes was carried out by the addition of 2 mM Mg-citrate at t0.5, and spores were harvested at t48. The plate assay was performed as described in Materials and Methods. The representative results for two independent clones from each strain are shown.