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. 2002 Nov;184(21):5999–6006. doi: 10.1128/JB.184.21.5999-6006.2002

FIG. 4.

FIG. 4.

Denaturing PAGE (12.5%) of cell extract of autotrophically grown C. aurantiacus and β-methylmalyl-CoA lyase at various steps of purification (20 μg each). Lanes: 1, molecular mass marker (rabbit phosphorylase b, 97 kDa; bovine serum albumin, 67 kDa; egg ovalbumin, 45 kDa; lactate dehydrogenase, 34 kDa; carboanhydrase, 29 kDa); 2, cell extract; 3, cell extract after heat precipitation; 4, DEAE-Sepharose fraction; 5, phenyl-Sepharose fraction; 6, size exclusion fraction; 7, Resource Q fraction. The gel was stained with Coomassie brilliant blue R-250. The arrow indicates a 38-kDa protein band that corresponds to β-methylmalyl-CoA lyase.