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. 2002 Nov;184(21):5971–5978. doi: 10.1128/JB.184.21.5971-5978.2002

FIG. 1.

FIG. 1.

Expression of txeR in E. coli. (A) E. coli strains carrying a txeR-ARU fusion were grown in broth and assayed for ARU production by ELISA (see Materials and Methods). The cells left the rapid-exponential-growth-phase after about 5 h. Solid circles, strain containing the TxeR-expressing plasmid pT7-txeR; open circles, strain carrying the vector pT7-7. (B) E. coli strains carrying txeR-ARU, toxA-ARU, and toxB-ARU fusions were grown in broth and harvested at the 20-h time point. Extracts were analyzed by Western blots with antibody to ARU. Lanes: 1, low-molecular-mass protein standards (kilodaltons); 2, no fusion plus pT7-7 vector; 3, toxA-ARU plus pT7-7; 4, toxA-ARU plus pT7-txeR; 5, toxB-ARU plus pT7-7; 6, toxB-ARU plus pT7-txeR; 7, txeR-ARU plus pT7-7; 8, txeR-ARU plus pT7-txeR; 9, no fusion plus pT7-txeR.