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. 2002 Nov;184(21):5971–5978. doi: 10.1128/JB.184.21.5971-5978.2002

FIG. 5.

FIG. 5.

Purification of TxeR. (A) SDS-PAGE analysis of protein extracts from E. coli −codon plus(DE3)-RIL carrying either pET28-b or pCD54. Lanes: 1, crude cell extract from E. coli carrying the vector pET28-b; 2, crude cell extract from E. coli carrying pCD54 (expressing TxeR); 3, soluble fraction of cell extract from E. coli carrying pCD54; 4, TxeR-His6 purified. GIBCO/BRL low-molecular-mass standards (kilodaltons) are shown adjacent to the gel. (B) Immunodetection of TxeR by using anti-TxeR antibody. The protein samples in each lane correspond to those in panel A.