TABLE 2.
Effects of deletion mutations in divJ on DivJ-GFP localization, growth, and viabilitya
Strain | Relevant genotype | DivJ-GFP foci at stalk poles (%)b | Doubling time (min) | Relative viability |
---|---|---|---|---|
CB15 | Parent strain | NA | 105 | 1.0 |
PC9808 | CB15/pTR100 | NA | 118 | 0.71 |
PC3017 | divJ::Ω | NA | 123 | 0.56 |
PC9809 | divJ::Ω/pTR100 | NA | 169 | 0.42 |
PC9810 | divJ::Ω/pdivJ-gfp | 72 | 124 | 0.68 |
PC9814 | divJ::Ω/pdivJ1-1668-gfp (DivJ1-557) | 75 | 116 | 0.60 |
PC9812 | divJ::Ω/pdivJ1-975-gfp (DivJ1-326) | 66 | 152 | 0.40 |
PC9813 | divJ::Ω/pdivJ1-735-gfp (DivJ1-245) | <1 | 168 | 0.40 |
PC9879 | divJ::Ω/pdivJΔ811-936 (DivJΔ271-312) | 4 | 124 | 0.62 |
PC9880 | divJ::Ω/pdivJΔ751-936 (DivJΔ251-312) | 2 | 123 | 0.62 |
Strains were grown and plated on PYE medium with antibiotics as appropriate. Results presented are the averages from three to five experiments. For DivJ-GFP scoring, over 200 cells were scored per strain. See Materials and Methods for details.
NA, not applicable.