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. 2002 Nov;184(21):6037–6049. doi: 10.1128/JB.184.21.6037-6049.2002

TABLE 2.

Effects of deletion mutations in divJ on DivJ-GFP localization, growth, and viabilitya

Strain Relevant genotype DivJ-GFP foci at stalk poles (%)b Doubling time (min) Relative viability
CB15 Parent strain NA 105 1.0
PC9808 CB15/pTR100 NA 118 0.71
PC3017 divJ::Ω NA 123 0.56
PC9809 divJ::Ω/pTR100 NA 169 0.42
PC9810 divJ::Ω/pdivJ-gfp 72 124 0.68
PC9814 divJ::Ω/pdivJ1-1668-gfp (DivJ1-557) 75 116 0.60
PC9812 divJ::Ω/pdivJ1-975-gfp (DivJ1-326) 66 152 0.40
PC9813 divJ::Ω/pdivJ1-735-gfp (DivJ1-245) <1 168 0.40
PC9879 divJ::Ω/pdivJΔ811-936 (DivJΔ271-312) 4 124 0.62
PC9880 divJ::Ω/pdivJΔ751-936 (DivJΔ251-312) 2 123 0.62
a

Strains were grown and plated on PYE medium with antibiotics as appropriate. Results presented are the averages from three to five experiments. For DivJ-GFP scoring, over 200 cells were scored per strain. See Materials and Methods for details.

b

NA, not applicable.