Fig. 11.

sEV-associated annexin A2 plays a major role in OTA-induced toxicity in NRK52E cells. A Expression of annexin A2 in silenced NRK52E cells-derived sEVs. An equal amount of sEV particles was lysed and loaded onto an SDS-PAGE gel. Ponceau staining was used for the loading control. B-C Immunoblotting of kidney injury markers in NRK52E cells treated with sEVs derived from knockdown cells. β-actin expression was used for normalization. D-E The expression of annexin A2 in Anxa2-GFP overexpression plasmid-transfected cells. TSG-101 expression was used for normalization. F Representative immunoblotting depicting the expression of kidney injury markers in NRK52E cells treated with sEVs of overexpressing cells. β-actin or α-tubulin expression was used for normalization. C-sEVs = NRK52E cells treated with sEVs derived from vehicle-treated NRK52E cells, Scr-sEVs = NRK52E cells treated with sEVs of scrambled-treated chronic OTA-exposed NRK52E cells, KD-sEVs = NRK52E cells treated with sEVs of annexin A2 siRNA-treated chronic OTA-exposed NRK52E cells, Vector = empty plasmid-transfected NRK52E cells, OE = Anxa2-GFP encoding plasmid-transfected NRK52E cells, Vec-sEVs = sEVs of empty plasmid-transfected NRK52E cells, OE-sEVs = sEVs of Anxa2-GFP encoding plasmid-transfected NRK52E cells