Table 1.
List of significantly upregulated proteins identified in the 250 nM OTA (LD)-induced sEVs vs control (C) sEVs group by LC/MS–MS analysis
| Accession | Description | Gene Symbol | Fold Change (Log2FC LDvsC) |
-Log (P- Value) |
|---|---|---|---|---|
| P62632 | Elongation factor 1-alpha 2 | Eef1a2 | 10.5 | 5.96E + 00 |
| Q63041 | Alpha-1-macroglobulin | A1m | 9.7 | 8.78E + 00 |
| P08650 | Complement C5 | C5 | 9.3 | 6.10E + 00 |
| P01346 | Insulin-like growth factor II | Igf2 | 8.9 | 5.63E + 00 |
| P16296 | Coagulation factor IX | F9 | 8.5 | 8.25E + 00 |
| P38652 | Phosphoglucomutase-1 | Pgm1 | 8.1 | 5.65E + 00 |
| Q5U300 |
Ubiquitin-like modifier-activating enzyme 1 |
Uba1 | 7.9 | 6.94E + 00 |
| P50399 | Rab GDP dissociation inhibitor beta | Gdi2 | 7.9 | 8.49E + 00 |
| O08651 |
D-3-phosphoglycerate dehydrogenase |
Phgdh | 7.8 | 7.42E + 00 |
| P11598 | Protein disulfide-isomerase A3 | Pdia3 | 7.6 | 5.15E + 00 |
| P55314 |
Complement component C8 beta chain |
C8b | 7.6 | 3.67E + 00 |
| P35444 | Cartilage oligomeric matrix protein | Comp | 7.6 | 4.49E + 00 |
| P62836 | Ras-related protein Rap-1A | Rap1a | 7.5 | 3.38E + 00 |
| Q3MHS2 | Zinc finger protein 830 | Znf830 | 7.4 | 7.41E + 00 |
| Q5XIM9 | T-complex protein 1 subunit beta | Cct2 | 7.4 | 4.92E + 00 |
| Q62894 | Extracellular matrix protein 1 | Ecm1 | 7.3 | 4.08E + 00 |
| P15800 | Laminin subunit beta-2 | Lamb2 | 7.1 | 3.82E + 00 |
| P04642 | L-lactate dehydrogenase A chain | Ldha | 7.0 | 3.09E + 00 |
| P06761 |
Endoplasmic reticulum chaperone BiP |
Hspa5 | 6.9 | 5.57E + 00 |
| P50503 | Hsc70-interacting protein | St13 | 6.8 | 5.43E + 00 |
| Q9WVC0 | Septin-7 | Septin7 | 6.8 | 6.35E + 00 |
| Q66HG4 | Galactose mutarotase | Galm | 6.8 | 6.78E + 00 |
| P61983 | 14–3-3 protein gamma | Ywhag | 6.8 | 4.78E + 00 |
| P10760 | Adenosylhomocysteinase | Ahcy | 6.7 | 2.14E + 00 |
| P05539 | Collagen alpha-1(II) chain | Col2a1 | 6.7 | 3.33E + 00 |
| P29534 | Vascular cell adhesion protein 1 | Vcam1 | 6.6 | 4.79E + 00 |
| P58751 | Reelin | Reln | 6.6 | 4.19E + 00 |
| P04762 | Catalase | Cat | 6.5 | 3.92E + 00 |
| Q66HD0 | Endoplasmin | Hsp90b1 | 6.5 | 3.91E + 00 |
| P62260 | 14–3-3 protein epsilon | Ywhae | 6.4 | 3.04E + 00 |
| O70199 | UDP-glucose 6-dehydrogenase | Ugdh | 6.4 | 4.29E + 00 |
| B3GNI6 | Septin-11 | Septin11 | 6.3 | 6.13E + 00 |
| P09811 | Glycogen phosphorylase, liver form | Pygl | 6.2 | 3.57E + 00 |
| P25113 | Phosphoglycerate mutase 1 | Pgam1 | 6.2 | 5.76E + 00 |
| P05197 | Elongation factor 2 | Eef2 | 6.2 | 4.32E + 00 |
| Q7TPB1 | T-complex protein 1 subunit delta | Cct4 | 6.2 | 6.49E + 00 |
| D3ZHA0 | Filamin-C | Flnc | 6.0 | 5.51E + 00 |
| P62804 | Histone H4 | H4c16;H4c2;Hist1h4m | 5.9 | 4.09E + 00 |
| Q9QZA2 |
Programmed cell death 6-interacting protein |
Pdcd6ip | 5.9 | 6.54E + 00 |
| P11442 | Clathrin heavy chain 1 | Cltc | 5.8 | 3.82E + 00 |
| Q6NYB7 | Ras-related protein Rab-1A | Rab1A | 5.7 | 2.63E + 00 |
| Q6RUV5 |
Ras-related C3 botulinum toxin substrate 1 |
Rac1 | 5.7 | 3.13E + 00 |
| Q8R2H2 | Integrin beta-3 | Itgb3 | 5.7 | 6.14E + 00 |
| Q07936 | Annexin A2 | Anxa2 | 5.6 | 2.84E + 00 |
| P25304 | Agrin | Agrn | 5.6 | 4.02E + 00 |
| Q9JLT0 | Myosin-10 | Myh10 | 5.6 | 4.46E + 00 |
| P13383 | Nucleolin | Ncl | 5.5 | 2.95E + 00 |
| P85968 | 6-phosphogluconate dehydrogenase, decarboxylating | Pgd | 5.5 | 3.03E + 00 |
| Q63610 | Tropomyosin alpha-3 chain | Tpm3 | 5.4 | 6.79E + 00 |
| P27653 |
C-1-tetrahydrofolate synthase, cytoplasmic |
Mthfd1 | 5.4 | 4.87E + 00 |
| P46462 |
Transitional endoplasmic reticulum ATPase |
Vcp | 5.2 | 3.78E + 00 |
| Q920P0 | L-xylulose reductase | Dcxr | 5.2 | 5.90E + 00 |
| P61589 | Transforming protein RhoA | Rhoa | 5.2 | 3.49E + 00 |
| Q63321 | Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 | Plod1 | 5.1 | 4.08E + 00 |
| Q08163 | Adenylyl cyclase-associated protein 1 | Cap1 | 5.0 | 5.24E + 00 |
| P46418 | Glutathione S-transferase alpha-5 | Gsta5 | 5.0 | 3.34E + 00 |
| Q4V7C7 | Actin-related protein 3 | Actr3 | 5.0 | 2.83E + 00 |
| P16638 | ATP-citrate synthase | Acly | 5.0 | 3.21E + 00 |
| P34058 | Heat shock protein HSP 90-beta | Hsp90ab1 | 4.9 | 4.76E + 00 |
| Q63347 | 26S proteasome regulatory subunit 7 | Psmc2 | 4.8 | 3.10E + 00 |
| O08618 |
Phosphoribosyl pyrophosphate synthase-associated protein 2 |
Prpsap2 | 4.7 | 1.92E + 00 |
| P12711 | Alcohol dehydrogenase class-3 | Adh5 | 4.6 | 2.82E + 00 |
| P68255 | 14–3-3 protein theta | Ywhaq | 4.6 | 2.80E + 00 |
| Q8CFN2 |
Cell division control protein 42 homolog |
Cdc42 | 4.5 | 2.17E + 00 |
| P47942 |
Dihydropyrimidinase-related protein 2 |
Dpysl2 | 4.5 | 3.43E + 00 |
| O35568 |
EGF-containing fibulin-like extracellular matrix protein 1 |
Efemp1 | 4.4 | 3.48E + 00 |
| P97603 | Neogenin (Fragment) | Neo1 | 4.4 | 4.07E + 00 |
| Q9Z1P2 | Alpha-actinin-1 | Actn1 | 4.4 | 3.40E + 00 |
| P05371 | Clusterin | Clu | 4.2 | 1.93E + 00 |
| P48500 | Triosephosphate isomerase | Tpi1 | 4.2 | 1.52E + 00 |
| P02454 | Collagen alpha-1(I) chain | Col1a1 | 4.0 | 4.97E + 00 |
| Q66HR2 |
Microtubule-associated protein RP/EB family member 1 |
Mapre1 | 3.9 | 2.63E + 00 |
| P85972 | Vinculin | Vcl | 3.8 | 4.68E + 00 |
| G3V928 |
Prolow-density lipoprotein receptor-related protein 1 |
Lrp1 | 3.8 | 2.36E + 00 |
| P82995 | Heat shock protein HSP 90-alpha | Hsp90aa1 | 3.6 | 6.12E + 00 |
| Q9WVC1 | Slit homolog 2 protein (Fragment) | Slit2 | 3.6 | 1.69E + 00 |
| P13084 | Nucleophosmin | Npm1 | 3.6 | 1.17E + 00 |
| O08628 |
Procollagen C-endopeptidase enhancer 1 |
Pcolce | 3.5 | 3.53E + 00 |
| Q9JLJ3 | 4-trimethylaminobutyraldehyde dehydrogenase | Aldh9a1 | 3.3 | 4.14E + 00 |
| P35952 | Low-density lipoprotein receptor | Ldlr | 3.2 | 2.00E + 00 |
| P62828 | GTP-binding nuclear protein Ran | Ran | 3.2 | 5.50E + 00 |
| P0DMW0 | Heat shock 70 kDa protein 1A | Hspa1a | 3.1 | 4.29E + 00 |
| Q5XIA3 |
tRNA wybutosine-synthesizing protein 4 |
Lcmt2 | 2.9 | 3.39E + 00 |
| Q91ZN1 | Coronin-1A | Coro1a | 2.8 | 3.21E + 00 |
| P63018 | Heat shock cognate 71 kDa protein | Hspa8 | 2.7 | 2.78E + 00 |
| P16617 | Phosphoglycerate kinase 1 | Pgk1 | 2.7 | 2.39E + 00 |
| P11980 | Pyruvate kinase PKM | Pkm | 2.7 | 6.16E + 00 |
| P63102 | 14–3-3 protein zeta/delta | Ywhaz | 2.6 | 3.09E + 00 |
| B0BNI5 | Olfactomedin-like protein 3 | Olfml3 | 2.6 | 3.36E + 00 |
| P04797 | Glyceraldehyde-3-phosphate dehydrogenase | Gapdh | 2.5 | 3.02E + 00 |
| Q64119 | Myosin light polypeptide 6 | Myl6 | 2.3 | 3.38E + 00 |
| O35303 | Dynamin-1-like protein | Dnm1l | 2.3 | 1.35E + 00 |
| O55096 | Dipeptidyl peptidase 3 | Dpp3 | 2.2 | 1.23E + 00 |
| P70490 | Lactadherin | Mfge8 | 2.1 | 1.77E + 00 |
| P0DP31 | Calmodulin-3 | Calm3 | 2.1 | 1.16E + 00 |
| P60711 | Actin, cytoplasmic 1 | Actb | 2.1 | 3.48E + 00 |
| P49744 | Thrombospondin-4 | Thbs4 | 2.1 | 3.13E + 00 |
| P02680 | Fibrinogen gamma chain | Fgg | 1.9 | 3.18E + 00 |
| Q7TMA5 | Apolipoprotein B-100 | Apob | 1.8 | 2.61E + 00 |
| P04764 | Alpha-enolase | Eno1 | 1.6 | 1.76E + 00 |
| P0C6B8 | Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1 | Svep1 | 1.4 | 1.09E + 00 |
No downregulated proteins were identified. Log2FC LDvsC represents the fold change in protein abundance in low-dose OTA-exposed sEVs relative to control sEVs. Significant deregulated proteins were identified if the adjusted p-value between experimental groups was ≤ 0.05 and the Log2FC was at least 1