Table 2.
List of significantly upregulated and downregulated proteins identified in the 500 nM OTA (HD) -induced sEVs vs control (C) sEVs group by LC/MS–MS analysis
| Accession | Description | Gene Symbol |
Fold Change (Log2FC HDvsC) |
-Log (P- Value) |
|---|---|---|---|---|
| P01346 | Insulin-like growth factor II | Igf2 | 11.0 | 6.88E + 00 |
| P62632 | Elongation factor 1-alpha 2 | Eef1a2 | 10.6 | 7.46E + 00 |
| Q63041 | Alpha-1-macroglobulin | A1m | 10.5 | 8.98E + 00 |
| Q5U300 | Ubiquitin-like modifier-activating enzyme 1 | Uba1 | 9.9 | 8.16E + 00 |
| P08650 | Complement C5 | C5 | 9.8 | 7.06E + 00 |
| P38652 | Phosphoglucomutase-1 | Pgm1 | 9.0 | 6.29E + 00 |
| P50399 | Rab GDP dissociation inhibitor beta | Gdi2 | 8.9 | 7.88E + 00 |
| P06761 | Endoplasmic reticulum chaperone BiP | Hspa5 | 8.7 | 7.93E + 00 |
| P16296 | Coagulation factor IX | F9 | 8.6 | 8.10E + 00 |
| Q62894 | Extracellular matrix protein 1 | Ecm1 | 8.4 | 4.62E + 00 |
| P15800 | Laminin subunit beta-2 | Lamb2 | 8.4 | 4.51E + 00 |
| Q3MHS2 | Zinc finger protein 830 | Znf830 | 8.3 | 8.02E + 00 |
| O08651 | D-3-phosphoglycerate dehydrogenase | Phgdh | 8.0 | 7.57E + 00 |
| Q66HD0 | Endoplasmin | Hsp90b1 | 8.0 | 5.94E + 00 |
| Q6RUV5 | Ras-related C3 botulinum toxin substrate 1 | Rac1 | 8.0 | 4.16E + 00 |
| P85968 |
6-phosphogluconate dehydrogenase, decarboxylating |
Pgd | 7.9 | 8.11E + 00 |
| B3GNI6 | Septin-11 | Septin11 | 7.8 | 7.31E + 00 |
| Q9WVC0 | Septin-7 | Septin7 | 7.7 | 6.85E + 00 |
| P58751 | Reelin | Reln | 7.7 | 4.79E + 00 |
| P25113 | Phosphoglycerate mutase 1 | Pgam1 | 7.6 | 6.51E + 00 |
| P05539 | Collagen alpha-1(II) chain | Col2a1 | 7.6 | 3.83E + 00 |
| P62804 | Histone H4 |
H4c16;H4c2; Hist1h4m |
7.6 | 7.46E + 00 |
| P35444 | Cartilage oligomeric matrix protein | Comp | 7.6 | 4.45E + 00 |
| P05197 | Elongation factor 2 | Eef2 | 7.5 | 4.86E + 00 |
| P61983 | 14–3-3 protein gamma | Ywhag | 7.5 | 5.20E + 00 |
| Q6NYB7 | Ras-related protein Rab-1A | Rab1A | 7.5 | 3.57E + 00 |
| Q07936 | Annexin A2 | Anxa2 | 7.4 | 4.24E + 00 |
| P25304 | Agrin | Agrn | 7.3 | 5.22E + 00 |
| Q63321 | Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 | Plod1 | 7.3 | 6.00E + 00 |
| P62836 | Ras-related protein Rap-1A | Rap1a | 7.3 | 3.26E + 00 |
| P10760 | Adenosylhomocysteinase | Ahcy | 7.2 | 2.32E + 00 |
| P04642 | L-lactate dehydrogenase A chain | Ldha | 7.2 | 3.20E + 00 |
| Q9WVC1 | Slit homolog 2 protein (Fragment) | Slit2 | 7.2 | 3.61E + 00 |
| Q7TPB1 | T-complex protein 1 subunit delta | Cct4 | 7.1 | 6.20E + 00 |
| O70199 | UDP-glucose 6-dehydrogenase | Ugdh | 7.0 | 4.72E + 00 |
| Q9QZA2 | Programmed cell death 6-interacting protein | Pdcd6ip | 7.0 | 7.71E + 00 |
| Q66HG4 | Galactose mutarotase | Galm | 7.0 | 6.98E + 00 |
| P27653 | C-1-tetrahydrofolate synthase, cytoplasmic | Mthfd1 | 7.0 | 6.01E + 00 |
| P12711 | Alcohol dehydrogenase class-3 | Adh5 | 7.0 | 6.10E + 00 |
| Q8R2H2 | Integrin beta-3 | Itgb3 | 6.9 | 7.14E + 00 |
| P11598 | Protein disulfide-isomerase A3 | Pdia3 | 6.8 | 4.64E + 00 |
| P46462 | Transitional endoplasmic reticulum ATPase | Vcp | 6.8 | 5.04E + 00 |
| P04762 | Catalase | Cat | 6.7 | 3.81E + 00 |
| P16638 | ATP-citrate synthase | Acly | 6.7 | 6.03E + 00 |
| P35952 | Low-density lipoprotein receptor | Ldlr | 6.7 | 4.88E + 00 |
| P62260 | 14–3-3 protein epsilon | Ywhae | 6.7 | 3.07E + 00 |
| P11442 | Clathrin heavy chain 1 | Cltc | 6.5 | 4.76E + 00 |
| P29534 | Vascular cell adhesion protein 1 | Vcam1 | 6.3 | 4.47E + 00 |
| Q4V7C7 | Actin-related protein 3 | Actr3 | 6.2 | 3.51E + 00 |
| P55314 | Complement component C8 beta chain | C8b | 6.1 | 3.02E + 00 |
| P97603 | Neogenin (Fragment) | Neo1 | 6.1 | 5.46E + 00 |
| P50503 | Hsc70-interacting protein | St13 | 6.1 | 4.85E + 00 |
| Q5XIM9 | T-complex protein 1 subunit beta | Cct2 | 6.1 | 2.55E + 00 |
| P48500 | Triosephosphate isomerase | Tpi1 | 6.1 | 4.57E + 00 |
| D3ZHA0 | Filamin-C | Flnc | 6.1 | 6.25E + 00 |
| P13383 | Nucleolin | Ncl | 5.9 | 2.85E + 00 |
| Q63610 | Tropomyosin alpha-3 chain | Tpm3 | 5.9 | 6.73E + 00 |
| O08618 | Phosphoribosyl pyrophosphate synthase-associated protein 2 | Prpsap2 | 5.8 | 2.48E + 00 |
| Q920P0 | L-xylulose reductase | Dcxr | 5.8 | 5.90E + 00 |
| Q9Z1P2 | Alpha-actinin-1 | Actn1 | 5.7 | 4.73E + 00 |
| P02454 | Collagen alpha-1(I) chain | Col1a1 | 5.6 | 7.52E + 00 |
| P09811 | Glycogen phosphorylase, liver form | Pygl | 5.5 | 3.00E + 00 |
| Q08163 | Adenylyl cyclase-associated protein 1 | Cap1 | 5.5 | 5.27E + 00 |
| P61589 | Transforming protein RhoA | Rhoa | 5.4 | 3.13E + 00 |
| P38650 | Cytoplasmic dynein 1 heavy chain 1 | Dync1h1 | 5.3 | 2.88E + 00 |
| B0BNI5 | Olfactomedin-like protein 3 | Olfml3 | 5.3 | 7.13E + 00 |
| P46418 | Glutathione S-transferase alpha-5 | Gsta5 | 5.3 | 2.91E + 00 |
| P05371 | Clusterin | Clu | 5.2 | 2.44E + 00 |
| P13084 | Nucleophosmin | Npm1 | 5.2 | 2.16E + 00 |
| O35568 |
EGF-containing fibulin-like extracellular matrix protein 1 |
Efemp1 | 5.2 | 3.99E + 00 |
| P47942 | Dihydropyrimidinase-related protein 2 | Dpysl2 | 5.2 | 3.44E + 00 |
| P34058 | Heat shock protein HSP 90-beta | Hsp90ab1 | 5.1 | 4.97E + 00 |
| Q6P9T8 | Tubulin beta-4B chain | Tubb4b | 5.0 | 3.51E + 00 |
| P0C6B8 |
Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1 |
Svep1 | 4.9 | 4.05E + 00 |
| O08628 | Procollagen C-endopeptidase enhancer 1 | Pcolce | 4.9 | 4.81E + 00 |
| Q8CFN2 | Cell division control protein 42 homolog | Cdc42 | 4.8 | 2.30E + 00 |
| P68255 | 14–3-3 protein theta | Ywhaq | 4.6 | 2.93E + 00 |
| Q63347 | 26S proteasome regulatory subunit 7 | Psmc2 | 4.6 | 2.74E + 00 |
| P0DMW0 | Heat shock 70 kDa protein 1A | Hspa1a | 4.4 | 6.50E + 00 |
| P63018 | Heat shock cognate 71 kDa protein | Hspa8 | 4.4 | 5.00E + 00 |
| Q9JLJ3 | 4-trimethylaminobutyraldehyde dehydrogenase | Aldh9a1 | 4.3 | 5.15E + 00 |
| P04797 | Glyceraldehyde-3-phosphate dehydrogenase | Gapdh | 4.2 | 6.40E + 00 |
| P11980 | Pyruvate kinase PKM | Pkm | 4.2 | 6.25E + 00 |
| O55096 | Dipeptidyl peptidase 3 | Dpp3 | 4.2 | 2.68E + 00 |
| P82995 | Heat shock protein HSP 90-alpha | Hsp90aa1 | 4.1 | 7.18E + 00 |
| P62828 | GTP-binding nuclear protein Ran | Ran | 4.0 | 6.71E + 00 |
| Q66HR2 | Microtubule-associated protein RP/EB family member 1 | Mapre1 | 4.0 | 2.66E + 00 |
| P85972 | Vinculin | Vcl | 4.0 | 4.60E + 00 |
| P16617 | Phosphoglycerate kinase 1 | Pgk1 | 4.0 | 4.37E + 00 |
| Q64119 | Myosin light polypeptide 6 | Myl6 | 3.7 | 5.64E + 00 |
| P49744 | Thrombospondin-4 | Thbs4 | 3.7 | 5.83E + 00 |
| G3V928 | Prolow-density lipoprotein receptor-related protein 1 | Lrp1 | 3.4 | 1.92E + 00 |
| Q5XIA3 | tRNA wybutosine-synthesizing protein 4 | Lcmt2 | 3.4 | 4.95E + 00 |
| P62982 | Ubiquitin-40S ribosomal protein S27a | Rps27a | 3.3 | 4.16E + 00 |
| Q7TMA5 | Apolipoprotein B-100 | Apob | 3.3 | 4.90E + 00 |
| Q9QWJ9 | Neuropilin-1 | Nrp1 | 3.3 | 1.75E + 00 |
| P04937 | Fibronectin | Fn1 | 3.2 | 6.05E + 00 |
| Q9EQT5 | Tubulointerstitial nephritis antigen-like | Tinagl1 | 3.2 | 5.41E + 00 |
| P60711 | Actin, cytoplasmic 1 | Actb | 3.2 | 7.39E + 00 |
| P04764 | Alpha-enolase | Eno1 | 3.1 | 7.09E + 00 |
| P63102 | 14–3-3 protein zeta/delta | Ywhaz | 3.1 | 4.02E + 00 |
| P70490 | Lactadherin | Mfge8 | 3.1 | 3.68E + 00 |
| Q9JLT0 | Myosin-10 | Myh10 | 3.1 | 2.68E + 00 |
| P69897 | Tubulin beta-5 chain | Tubb5 | 3.0 | 6.27E + 00 |
| Q91ZN1 | Coronin-1A | Coro1a | 2.9 | 3.16E + 00 |
| Q62812 | Myosin-9 | Myh9 | 2.9 | 6.11E + 00 |
| P02680 | Fibrinogen gamma chain | Fgg | 2.9 | 5.37E + 00 |
| Q5XIF6 | Tubulin alpha-4A chain | Tuba4a | 2.6 | 5.17E + 00 |
| Q8CHN8 | Mannan-binding lectin serine protease 1 | Masp1 | 2.5 | 4.40E + 00 |
| P63259 | Actin, cytoplasmic 2 | Actg1 | 2.3 | 3.46E + 00 |
| O35303 | Dynamin-1-like protein | Dnm1l | 2.1 | 1.13E + 00 |
| P08721 | Osteopontin | Spp1 | 2.0 | 1.46E + 00 |
| Downregulated proteins | ||||
| P15865 | Histone H1.4 | H1-4 | −2.3 | 2.35E + 00 |
Log2FC HDvsC represents the fold change in protein abundance in high-dose OTA-induced sEVs relative to control sEVs. Significantly deregulated proteins were identified if the adjusted p-value between experimental groups was ≤ 0.05 and the Log2FC was at least 1