Table 3.
All significantly upregulated and downregulated proteins in urinary sEVs derived from OTA-exposed rats (T) vs vehicle-control (VC) sEVs
| Accession | Description | Gene Symbol |
Fold Change (Log2FC TvsVC) | -Log (P-value) |
|---|---|---|---|---|
| P14841 | Cystatin-C | Cst3 | 11.6 | 3.70E + 00 |
| P81828 | Urinary protein 2 | 10.9 | 3.09E + 00 | |
| P20059 | Hemopexin | Hpx | 9.9 | 3.20E + 00 |
| P06866 | Haptoglobin | Hp | 9.8 | 2.37E + 00 |
| P42854 | Regenerating islet-derived protein 3-gamma | Reg3g | 9.6 | 3.40E + 00 |
| P24090 | Alpha-2-HS-glycoprotein | Ahsg | 9.1 | 2.55E + 00 |
| A0A0G2JZV7 | Uncharacterized protein | 8.8 | 3.32E + 00 | |
| Q7TMB9 | Ab1-021 | Serpina3l | 8.6 | 3.22E + 00 |
| Q5PQU1 | Kininogen 1 | Kng2 | 8.3 | 4.61E + 00 |
| Q6IN22 | Cathepsin B | Ctsb | 8.2 | 3.73E + 00 |
| P27274 | CD59 glycoprotein | Cd59 | 8.0 | 3.31E + 00 |
| M0RBP7 | Uncharacterized protein | 7.9 | 3.05E + 00 | |
| M0R4Z4 | Uncharacterized protein | 7.9 | 2.44E + 00 | |
| D3ZEP5 | Uncharacterized protein | 7.5 | 3.83E + 00 | |
| Q07936 | Annexin A2 | Anxa2 | 7.5 | 2.76E + 00 |
| A0A0G2JZ73 | Alpha-1-antiproteinase | 6.9 | 3.49E + 00 | |
| Q64602 | Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial | Aadat | 6.7 | 3.15E + 00 |
| F7EPE0 | Prosaposin | 6.7 | 2.48E + 00 | |
| Q5VLR6 | BWK3 | 6.7 | 1.95E + 00 | |
| P81827 | Urinary protein 1 | 6.6 | 5.56E + 00 | |
| Q62930 | Complement component C9 | C9 | 6.5 | 2.88E + 00 |
| P10959 | Carboxylesterase 1C | Ces1c | 6.5 | 2.28E + 00 |
| Q01177 | Plasminogen | Plg | 6.4 | 1.24E + 00 |
| P02680 | Fibrinogen gamma chain | Fgg | 6.4 | 2.96E + 00 |
| M0RBK4 | Uncharacterized protein | 6.4 | 3.15E + 00 | |
| E0A3N4 | Serpina3n-like protein | 6.3 | 1.95E + 00 | |
| A0A0G2K4C9 | Prostatic spermine-binding protein | Sbp | 6.1 | 2.05E + 00 |
| E9MW47 | Immune CD300 receptor | Cd300le | 5.8 | 1.69E + 00 |
| A0A0G2JSH5 | Serum albumin | Alb | 5.8 | 5.92E + 00 |
| P12346 | Serotransferrin | Tf | 5.7 | 4.34E + 00 |
| G3V9M0 | Cystatin | Cyssl1 | 5.5 | 3.15E + 00 |
| A0A0G2K7I1 | Uncharacterized protein | 5.4 | 5.18E + 00 | |
| A0A0G2K6T8 | Uncharacterized protein | 5.4 | 2.91E + 00 | |
| A0A0G2K926 | Murinoglobulin-1 | LOC297568 | 5.1 | 4.22E + 00 |
| P20767 | Ig lambda-2 chain C region | 5.1 | 3.84E + 00 | |
| P04276 | Vitamin D-binding protein | Gc | 5.1 | 2.48E + 00 |
| P36953 | Afamin | Afm | 4.8 | 1.29E + 00 |
| A0A0G2K4K2 | Uncharacterized protein | 4.7 | 1.83E + 00 | |
| F1LTN6 | Uncharacterized protein | 4.7 | 3.76E + 00 | |
| M0RD98 | Uncharacterized protein | 4.5 | 3.07E + 00 | |
| P83121 | Urinary protein 3 | 4.5 | 4.05E + 00 | |
| Q9JJI4 | Alpha-2u globulin | Mup4l1 | 4.3 | 3.06E + 00 |
| G3V8G8 | Kallikrein 6 (Predicted) | Klk1c8 | 4.3 | 3.61E + 00 |
| Q4VBH1 | Ighg protein | Ighg | 4.1 | 1.25E + 00 |
| P02770 | Serum albumin | Alb | 4.0 | 3.41E + 00 |
| D3ZQR5 | Uncharacterized protein | 4.0 | 4.84E + 00 | |
| Q63430 | Cold shock domain-containing protein C2 | Csdc2 | 3.9 | 1.95E + 00 |
| G3V8H1 | Kallikrein 1-related peptidase B3 | Klk1 | 3.8 | 1.71E + 00 |
| P01015 | Angiotensinogen | Agt | 3.6 | 1.99E + 00 |
| F1LPG1 | Uncharacterized protein | 3.4 | 3.48E + 00 | |
| Q5I0L0 | Alpha-amylase | Amy1a | 3.3 | 4.20E + 00 |
| M0RBF1 | Complement C3 | C3 | 3.2 | 3.46E + 00 |
| A0A0G2JXF0 | Uncharacterized protein | 3.1 | 2.13E + 00 | |
| P21704 | Deoxyribonuclease-1 | Dnase1 | 3.0 | 2.72E + 00 |
| Q63530 | Phosphotriesterase-related protein | Pter | 3.0 | 1.67E + 00 |
| P15399 | Probasin | Pbsn | 3.0 | 3.47E + 00 |
| P05545 | Serine protease inhibitor A3K | Serpina3k | 2.9 | 1.75E + 00 |
| H6X320 | Pentaxin | Apcs | 2.9 | 2.52E + 00 |
| A0A0G2JSP1 | Uromodulin | 2.7 | 3.45E + 00 | |
| A0A0G2JUY4 | Uncharacterized protein | 2.7 | 2.54E + 00 | |
| P02780 | Secretoglobin family 2 A member 2 | Scgb2a2 | 2.6 | 4.63E + 00 |
| A0A0G2JV65 | 14–3-3 protein zeta/delta | 2.5 | 1.81E + 00 | |
| Q5M7V3 | LOC367586 protein | LOC367586 | 2.4 | 2.70E + 00 |
| A0A1W2Q6M4 | Uncharacterized LOC103691699 (Fragment) | 2.3 | 3.86E + 00 | |
| Q63015 | Common salivary protein 1 | Csap1 | 2.1 | 1.80E + 00 |
| Q6IE67 | Proteasome subunit alpha type | LOC100361067 | 2.0 | 1.13E + 00 |
| Downregulated proteins | ||||
| D3ZUQ1 | Lipase | Lipo1 | −8.4 | 2.61E + 00 |
| P07314 | Glutathione hydrolase 1 proenzyme | Ggt1 | −7.1 | 2.10E + 00 |
| Q64319 | Neutral and basic amino acid transport protein rBAT | Slc3a1 | −7.0 | 3.16E + 00 |
| A0A0H2UHX5 | Neprilysin | Mme | −7.0 | 2.34E + 00 |
| P10111 | Peptidyl-prolyl cis–trans isomerase A | Ppia | −5.9 | 3.01E + 00 |
| Q9QZT0 | CUB and zona pellucida-like domain-containing protein 1 | Cuzd1 | −5.5 | 1.64E + 00 |
| D3Z9U8 | S100 calcium-binding protein A7-like 2 | S100a7l2 | −5.4 | 2.10E + 00 |
| F1M9X2 | Pancreatic secretory granule membrane major glycoprotein GP2 | Gp2 | −4.6 | 1.07E + 00 |
| Q05175 | Brain acid soluble protein 1 | Basp1 | −4.2 | 1.86E + 00 |
| A0A0G2K1A2 | Myeloperoxidase | −4.2 | 2.93E + 00 | |
| P31977 | Ezrin | Ezr | −4.1 | 1.87E + 00 |
| Q4QQV8 | Charged multivesicular body protein 5 | Chmp5 | −3.1 | 2.85E + 00 |
| Q91XN5 | Prominin 1 | Prom1 | −2.9 | 2.18E + 00 |
| P15684 | Aminopeptidase N | Anpep | −2.7 | 1.54E + 00 |
| P63259 | Actin, cytoplasmic 2 | Actg1 | −2.4 | 1.69E + 00 |
| F1MAE5 | Uncharacterized protein | −2.3 | 2.59E + 00 | |
| Q45QL6 | Guanine nucleotide binding protein beta 2 (Fragment) | Gnb2 | −2.1 | 1.22E + 00 |
| P50123 | Glutamyl aminopeptidase | Enpep | −1.9 | 1.59E + 00 |
| D3ZS19 | Alpha-2-macroglobulin-like 1 | −1.6 | 1.32E + 00 | |
| P0DP31 | Calmodulin-3 | Calm3 | −1.1 | 1.78E + 00 |
Log2FC TvsVC represents the fold change in protein abundance in high-dose OTA-induced sEVs relative to vehicle control-derived sEVs Significantly deregulated proteins were identified if the adjusted p-value between experimental groups was ≤ 0.05 and the Log2FC was at least 1