FIG. 2.

Schematic of the cI gene denoting subcloned fragments and their effect on phage φ31. Lines indicate regions subcloned into pTRKH2. EOPs and plaque sizes in millimeters are for phage φ31 plated on NCK203 containing the subclones. EOPs for the identical fragments cloned into pAK80 are given in brackets. The ∗ indicates the point mutation C to T that truncated the CI protein. a, full-length wild-type pTRHH2::cI NCK203 transformant was not obtained; b, the pTRKH2 subclone could not be obtained in E. coli.