TABLE 1.
State and day | Measure of viability
|
|||
---|---|---|---|---|
No. of cell particles/mla | No. of CFU/ml | % Live cellsb | Presence of PBP5 mRNAc | |
Starvation | ||||
0 | 1 × 107 | 1 × 107 | 100 | + |
10 | 1.5 × 107 | 3 × 106 | 91 | + |
20 | 9 × 106 | 2 × 106 | 83 | + |
VBNC | ||||
0 | 1.5 × 107 | 1 × 107 | 100 | + |
10 | 9 × 106 | 1 × 102 | 86 | + |
20 | 8.5 × 106 | <0.1 | 70 | + |
Determined with a Coulter counter.
Determined by Live/Dead staining kit.
Determined by RT-PCR. PBP5, penicillin binding protein 5.