TABLE 3.

RT-PCR analysis of VBNC E. faecalis cells

Gene	Protein	Primers	Amplicon (bp)	mRNA detected in VBNC cells
				Day 0	Day 7	Day 14
fba	Fructose-bisphosphate aldolase	5′ GCAGCAGAAGCTAA 3′	270	+	+	±
		5′ AGATACCTTTAGCGTG 3′
tsf	EF-Ts	5′ TCTTCCCCTTGGTTACC 3′	180	+	+	+
		5′ CGTAAAGAACGCATTGC 3′
tuf	EF-Tu	5′ TGGCCCTATGCCTCAAAC 3′	400	+	−	−
		5′ ACCAGTTGCAACAGTACC 3′
pbp5	Penicillin binding protein 5	5′ CATGCGCAATTAATCGG 3′	444	+	+	+
		5′ CATAGCCTGTCGCAAAAC 3′
ftsz	FTSZ	5′ AAGTTATCGGTGTAG 3′	400	±	−	−
		5′ GCGGCAAAACGACCA 3′