FIG. 1.
Genetic map of ISPpu12. The location and orientation of ISPpu12 relative to the xyl genes of the meta pathway operon on plasmid pWW0-1216, from which it was first isolated, are shown at the top. The bases are numbered according to GenBank accession numbers AJ344068 (complete pWW0 sequence) and AY128707 (ISPpu12). The sequences of the two terminal inverted repeats are given below the map. The location of the Kmr cassette in the unique KpnI site between orf1 and orf2 to form ISPpu12-Km on plasmids pGPTMKm1 and pGPTKm2 is indicated. aa, amino acids.