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. 2002 Dec;184(23):6572–6580. doi: 10.1128/JB.184.23.6572-6580.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 4. — Southern blots of digests of DNAs from P. putida mt-2 and Pseudomonas sp. strain B13 probed with the orf1-specific probe generated from ISPpu12. (A) Plasmid DNAs. Lane 1, plasmid pXQ3Km2 control; lanes 2, 5, and 8, pWW0; lanes 3, 6, and 9, pWW0-1216-2; lanes 4, 7, and 10, pWW0-1216-3. Lanes S, digestion with SalI; lanes SE, digestion with SalI and EcoRI; lanes E, digestion with EcoRI. Neither SalI nor EcoRI cuts within ISPpu12. (B) SalI digests of genomic DNAs. Lane 1, P. putida mt-2; lane 2, Pseudomonas sp. strain B13; lane 3, Pseudomonas sp. strain WR216-2; lane 4, Pseudomonas sp. strain WR216-3.