FIG. 5.

Relationship between ISPpu12 and other reported sequences. (A) Location of the 355 bp containing the constitutive promoter, firing from right to left, on pTN8 reported by Inouye et al. (12). (B) Postulated IS1396 from S. marcescens plasmid R471a (16). The map of IS1396 is drawn from the sequence under accession number AF027768 and is proposed to carry a tnpA gene between two terminal inverted repeats (IRs) (27 of 49 bp), shown on the map as blunt arrows with their sequences below. The area of the AF027768 sequence corresponding to 100% homology with ISPpu12 is indicated by the hatched area spanning tnpA and running to the left to include an ORF identical to lspA. There is no similarity between ISPpu12 and the nucleotide sequence on R451a to the right of IRL.