FIG. 3.

Western immunoblot of crude extracts from photoheterotrophically grown R. sphaeroides strains using form I PRK antiserum, which cross-reacts with both form I and II PRKs. Shown here is a scanned image of the original colorimetric blot. Form I PRK is ∼32 kDa, and form II PRK is ∼34 kDa. Lane 1, E. coli JM109 containing pJN940A (form I PRK); lane 2, E. coli JM109 containing pJN1050BS (form II PRK); lane 3, wild-type strain HR; lane 4, strain 16; lane 5, strain 16PHC; lane 6, strain 16PHG. These extracts were from strains grown with 30 mM DMSO in the medium. Ten micrograms of protein was loaded in each lane.