TABLE 2.
Oligonucleotides used in this study
| Primer | Sequence | Positiona |
|---|---|---|
| E9 | 5′ ACT GTG TTT CGG GTA GC | 1722-1738 |
| E12 | 5′ TAT GGG AGT TGT GAA | 2711-2725 |
| E15 | 5′ CAG AAG CTG AGC TAG T | 2576-2561 |
| E35 | 5′ GCG AGC TCA CAG ATC AGG AAA TCG GAb | NAc |
| E37 | 5′ GGA TCA CCG AAG AAG GT | 119-135 |
| E38 | 5′ CCA GGC ATT GTA TTG ATC T | 932-914 |
| E41 | 5′ GCA ATT GCT AAC CCT AGA CC | 5464-5445 |
| E43 | 5′ GCG AGC TCA ACA AAT ACT GGA GGT Ab | NA |
| XYE1 | 5′ GTT CAG GCT CCG TTT GCG C | 1103-1121 |
| XYE2 | 5′ GCA GTT CCT CTT ATT GAC TC | 1599-1580 |
| TE4 | 5′ GCA GCG GTT CAG GTG TTT | 2159-2174 |
| TE5 | 5′ GCG CAT GCA GCC ATT AAA CAT CCTd | 3785-3770 |
| RE1 | 5′ CCG AGA CAG CCA AAT | 4410-4424 |
| RE2 | 5′ TCG ACT GTC GAC AAA T | 4214-4199 |
| RE6 | 5′ AGA TCG ATT TAG CCA TAC | 3913-3930 |
| SE1 | 5′ AGC TAA CAG ATT AGA ACA T | 5027-5045 |
| SE2 | 5′ GGA GTT CTT AAG TCA TGT | 4921-4904 |
| VDV | 5′ GAT CGT TGG TTT TTA GAT | 178-195 |
| RDeg2 | 5′ CCN ACH CCS CRB ACV GTTe | NA |
| PE1 | 5′ CCA ATG ACC TTC TTC GGT GAT CC | 120-96 |
| PE3 | 5′ AAG CTT TCT TTT CCT GAC ATA GCC TC | 3877-3851 |
| SDEg1 | 5′ ATS GSM ARH CCM ARW CCf | NA |
a
Nucleotide numbering begins at the first base of the vanE gene.
b
The SacI site is underlined.
c
NA, not applicable.
d
The SphI site is underlined.
e
B = C, G, or T; H = A, C, or T; N = A, C, G, or T; R = A or G; S = G or T; V = A, C, or G.
f
H = A, C or T; M = A or C; R = A or G; S = G or T; W = A or T.