TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Genotype | Source or reference |
|---|---|---|
| R. sphaeroidesa | ||
| HR | Wild type; Smr | 33 |
| PrrA | HRCAC, prrA::SalI spc cartridge of pHP45 inserted into XhoI site of prrA | Y. Qian and F. R. Tabita, unpublished results |
| HRΩ | HRCAC prrB::ΩSp | 24 |
| 2.4.1 | Wild type | |
| CcoP1 | 2.4.1 ccoP::ΩTp | 18 |
| CcoP1PrrA | 2.4.1 ccoP::ΩTprrA::ΩSpc, | 19 |
| CcoQΔ | 2.4.1, in-frame deletion in ccoQ | 22 |
| R. capsulatus | ||
| MT1131 | Wild type, crtD121 Rifr | 27 |
| GK32 | MT1131 ccoNO::Km cox | 14 |
| MG1 | MT1131 ccoP::Km cox | 14 |
| M4 | MT1131 ΔccoN | 27 |
| Plasmids | ||
| pVKC1 | pVK102 Tcr Kmr, cbbI::lacZYA | 2 |
| pVKCII | pVK102 Tcr Kmr, cbbII::lacZYA | This study |
a
All R. sphaeroides strains except CCOQΔ are CAC.