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. 2002 Dec;184(23):6472–6480. doi: 10.1128/JB.184.23.6472-6480.2002

FIG. 3.

FIG. 3.

Complementation of Pqs− mutants. PQS samples extracted from 24-h cultures of strains carrying different pqs genes in plasmids were analyzed by TLC. (A) Lane 1, strain MPAO1 grown without a plasmid; lanes 2 to 7, strains MPAO1, MP703 (pqsC), MP704 (pqsD), MP605 (pqsE), MP710 (phnA), and MP551 (mvfR), respectively, carrying plasmid pUCP18; lanes 8 to 13, the same strains as the previous six lanes, but carrying plasmid pLG10; lane 14, 50 ng of PQS. (B) Lane 1, strain MPAO1 grown without a plasmid; lanes 2 to 7, strains MPAO1, MP703 (pqsC), MP704 (pqsD), MP605 (pqsE), MP710 (phnA), and MP551 (pqsR), respectively, carrying plasmid pLG12; lanes 8 to 10, strains MPA01, MP703 (pqsC), and MP704 (pqsD), respectively, carrying plasmid pLG14; lanes 11 to 14, strains MPAO1, MP703 (pqsC), MP704 (pqsD), and MP605 (pqsE), respectively, carrying plasmid pLG16; lane 15, 50 ng of PQS. The arrowhead indicates the position of PQS. (C) Summary of complementation results shown in panels A and B.