TABLE 2.
β-Galactosidase activities in strains carrying chromosomal lacZ reporter fusions to quorum-sensing-controlled genesa
| Mutant gene |
phzC1-lacZ
|
hcnB-lacZ
|
pqsH-lacZ
|
phzD2-lacZ
|
rhlI-lacZ
|
cytC-lacZ
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| % Activity | (SEM) | nc | % Activity | (SEM) | n | % Activity | (SEM) | n | % Activity | (SEM) | n | % Activity | (SEM) | n | % Activity | (SEM) | n | |
| w-t | (100) | (8) | 13b | (100) | (8) | 12b | (100) | (3) | 10b | (100) | (6) | 12b | (100) | (2) | 9b | (100) | (8) | 10b |
| pqsC | 16 | (3) | 3 | 43 | (4) | 3 | 85 | (4) | 2 | 117 | (16) | 2 | 91 | (5) | 2 | 71 | (13) | 2 |
| pqsD | 4 | (0) | 3 | 30 | (5) | 2 | 82 | (1) | 2 | 140 | (9) | 2 | 84 | (7) | 2 | 65 | (10) | 2 |
| pqsE | 3 | (0) | 3 | 30 | (3) | 2 | 84 | (2) | 2 | 121 | (11) | 2 | 88 | (4) | 4b | 74 | (12) | 2 |
| phnA | 14 | (2) | 3 | 38 | (3) | 2 | 83 | (4) | 2 | 108 | (20) | 2 | 89 | (6) | 2 | 75 | (17) | 2 |
| pqsR | 5 | (1) | 8b | 27 | (0) | 5b | 80 | (2) | 5b | 148 | (14) | 4b | 88 | (3) | 5b | 76 | (9) | 3 |
| pqsH | 8 | (2) | 5b | 32 | (1) | 3 | NDd | 128 | (14) | 5b | 84 | (4) | 4b | 72 | (6) | 5b | ||
| lasR | 1 | (0) | 3 | 7 | (2) | 8b | 9 | (2) | 3 | 5 | (1) | 4b | 5 | (0) | 4b | 49 | (4) | 4b |
| np20 | ND | 6 | (1) | 6b | 6 | (0) | 6b | 4 | (0) | 7b | 5 | (0) | 6b | 61 | (2) | 7b | ||
| PA3946 | 162 | (17) | 8b | 20 | (1) | 5b | 47 | (1) | 4b | 85 | (4) | 7b | 58 | (1) | 4b | 64 | (5) | 3 |
a
The chromosomal qsc-gene-lacZ reporter alleles tested are listed at the top of each data column. The gene mutated in each strain is indicated in the far left column. β-Galactosidase activity is shown for each reporter allele as a percentage of the average activity in the wild-type background. 100% β-galactosidase activity represents, for phzC1-lacZ, 813 Miller units; for hcnB-lacZ, 534 units; for pqsH-lacZ, 268 units; for phzD2-lacZ, 77 units; for rlhI-lacZ, 2,000 units; and for cytC-lacZ, 95 units.
b
Value determined from analysis of duplicate constructed strains.
c
n, number of independent assays performed.
d
ND, not done.