TABLE 3.
Induction of phzC1-lacZ expression by extracellular complementation
| Adjacent straina |
phzC1-lacZ fusion straina,b
|
||||||
|---|---|---|---|---|---|---|---|
| MP882.1 (pqsC) | MP883.1 (pqsD) | MP884.1 (pqsE) | MP894.1 (phnA) | MP885.2 (pqsR) | MP886.1 (pqsH) | MP892.1 (lasR) | |
| MPAO1 (wild-type) | + | ++ | − | + | +/− | +++ | ++++ |
| MP703 (pqsC) | − | − | − | − | − | − | − |
| MP704 (pqsD) | − | − | − | − | − | + | − |
| MP605 (pqsE) | + | ++ | − | + | +/− | +++ | ++++ |
| MP710 (phnA) | − | − | − | − | − | − | − |
| MP551 (pqsR) | − | − | − | − | − | − | +/− |
| MP562 (pqsH) | + | + | − | +/− | +/− | − | +++ |
| MP701 (lasR) | +/− | + | − | − | − | − | − |
| MP607 (rhlR/I) | +/− | ++ | − | − | − | +++ | ++++ |
| MP611 (np20) | − | − | − | − | − | − | − |
a
The gene mutated in each strain is indicated paranthetically
b
Increased β-galactosidase activity over background levels as measured by X-Gal hydrolysis was assessed visually and ranged from slight (+/−) to extensive (++++).