TABLE 1.
Mutagenesis of the conserved sequence at the 5′ end of the pyrG leader
| Strain | Mutation | Mutation position | β-Galactosidase activity (Miller units)
|
||
|---|---|---|---|---|---|
| +Cytidine | +Orotate | Ratio | |||
| QM401 | None | 40 ± 4 | 600 ± 34 | 15 | |
| QM403 | GGG to AAA | +1 to +3 | <1 | <1 | |
| QM416 | GGG to AGG | +1 | <1 | 1.4 ± 0.6 | |
| QM404 | GGG to GAG | +2 | 5 ± 0.4 | 1.3 ± 0.2 | 0.3 |
| QM405 | GGG to GGA | +3 | <1 | <1 | |
| QM412 | C to A | +4 | 100 ± 36 | 200 ± 51 | 2 |
| QM413 | C to U | +4 | 14 ± 2 | 550 ± 92 | 39 |
| QM417 | U to G | +5 | 34 ± 2 | 1,000 ± 300 | 29 |
| QM417C | U to C | +5 | 30 ± 2 | 700 ± 140 | 23 |
| QM414 | AAA +1 insertion | 12 ± 3 | 90 ± 21 | 8 | |
| QM408 | QM405 with terminator deletion | 150 ± 30 | 300 ± 50 | 2 | |