FIG. 3.

The McpB protein is degraded during the swarmer-to-stalked-cell transition. (A) Strain MRKA1374 bearing the xylose inducible mcpB gene was grown in PYE medium supplemented with glucose or xylose, and extracts from equal amount of cell material as calculated by A₆₀₀ were analyzed for presence of McpB by immunoblotting with McpA antisera. (B) Strain MRKA1374 was grown in PYEX before cells were synchronized and allowed to proceed through the cell cycle in the same medium. Extract of equal amounts of cell material were analyzed by immunoblotting with McpA antisera. One cell cycle unit was 100 min. (C) Strain MRKA1374 was grown in PYEX before synchronization and was then allowed to proceed through the cell cycle in PYEG. Cell extracts from equal culture volumes of a synchronous population of MRKA1374 were analyzed for the presence of McpB by immunoblotting with McpA antisera. One cell cycle unit was 100 min.