FIG. 1.
EMSA with C. trachomatis dnaK CIRCE probe and recombinant HrcA. Positions of free and bound probes are marked. (A) Lane 1 (counting from the left), labeled probe alone; lanes 2 to 5, labeled probe with addition of twofold-increased concentrations of HrcA. (B) Lane 1, labeled probe alone; lane 2, addition of HrcA alone; lane 3, addition of HrcA and polyclonal anti-HrcA antibody; lane 4, addition of polyclonal anti-HrcA antibody alone. All reactions were performed with a 0.5 nM 32P-labeled dnaK CIRCE probe.