Skip to main content
. 2002 Dec;184(23):6566–6571. doi: 10.1128/JB.184.23.6566-6571.2002

FIG. 3.

FIG. 3.

Competitive EMSA with C. trachomatis dnaK CIRCE probes and purified recombinant HrcA. (A) Representation of DNA fragments used in EMSA. CIRCE and promoter elements are marked. (B) Competition with CIRCE-containing restriction fragments. Lane 1, probe alone; lane 2, no competitor; lanes 3 to 7, molar excess of specific competitor relative to labeled probe (2-, 4-, 8-, 16-, and 32-fold); lanes 8 to 12, molar excess of nonspecific competitor (2-, 4-, 8-, 16-, and 32-fold). (C) Competition with CIRCE-containing oligonucleotides. Lane 1, probe alone; lane 2, no competitor; lanes 3 to 6, molar excess of specific competitor (4-, 8-, 16-, and 32-fold); lanes 7 to 10, molar excess of nonspecific competitor (4-, 8-, 16-, and 32-fold). All reactions, with the exception of reactions lacking HrcA, were performed with 0.5 nM labeled probe and 160 nM HrcA.